Unleashing the Power of Polar Separations
Hydrophilic interaction chromatography (HILIC) is a powerful chromatographic technique for separating polar compounds. It offers a unique selectivity compared to reversed-phase chromatography, making it ideal for a wide range of applications, from the analysis of polar metabolites and carbohydrates to the separation of peptides and proteins.
PolyLC has been at the forefront of HILIC development since its inception. Our founder, Dr. Andrew Alpert, is credited with inventing and pioneering this essential chromatographic technique. We offer a comprehensive range of HILIC columns, including our flagship PolyHYDROXYETHYL A™ and the HILICON iHILIC® columns, to provide you with the tools you need to achieve superior separations for your polar analytes.
In HILIC, a hydrophilic stationary phase attracts polar molecules in the sample. Polar molecules in the sample interact with and are retained by the stationary phase. Less polar molecules elute first, followed by more polar molecules, as the concentration of the organic solvent decreases.
PolyHYDROXYETHYL A™: The Original HILIC Material
PolyHYDROXYETHYL A™ is our flagship HILIC material, offering exceptional performance and versatility for the separation of polar compounds. Its unique hydrophilic coating provides strong retention of polar analytes, while its compatibility with a wide range of mobile phases allows for fine-tuning selectivity to achieve optimal separations.
Key Features:
- Reduced Solvent: The high polarity of PolyHYDROXYETHYL A™ allows for effective analyte retention with lower organic solvent concentrations in your mobile phase
- Solubility: Enables HPLC analysis of solutes that are not soluble in aqueous media, such as membrane proteins and phospholipids
- Performance: Compared to other HILIC materials, PolyHYDROXYETHYL A™ provides sharper peaks, better selectivity, and higher recovery of your valuable analytes.
Other HILIC Materials
PolyLC offers a variety of other HILIC materials, including:
- PolyCAT A: A strong cation exchange material that can be used in HILIC mode for the separation of basic peptides.
- PolySULFOETHYL A: A strong anion exchange material that can be used in HILIC mode for the separation of acidic peptides.
- PolyWAX LP: A weak anion exchange material that can be used in HILIC mode for the separation of polar compounds.
Select the pore diameter that matches your application:
- 60- or 100-Å: Small solutes in general. See Metabolomics. The 3-µm, 100-Å material is particularly useful.
- 200- or 300-Å: Peptides and most proteins; di- and trinucleotides (ADP; NADH; etc.)
- 1000-Å: Extremely polar solutes such as ATP and aminoglycoside antibiotics (see Metabolomics)
FEATURED APPLICATION NOTE:
Optimize Polar Separations with HILIC
Learn how to optimize HILIC separations using PolyHYDROXYETHYL A™. This resource provides insights into how mobile phase composition and solvent type influence elution order and selectivity. Discover strategies for separating polar compounds, including peptides with post-translational modifications, and minimizing interference from sample matrices.
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