Struggling with Complex Separations and Process Variability?

Isolating and purifying your analyte of interest can be a real challenge. Traditional reversed-phase chromatography often falls short of achieving optimal analyte resolution from complex mixtures, while demanding applications and low-quality columns can introduce variability into experimental results, bringing research to a halt. 

PolyLC provides innovative chromatography solutions, including HILIC columns and a portable HPLC instrument, that are designed to overcome complex separation challenges. Our high-quality products are carefully manufactured to provide consistent performance and our experienced team can help you design custom solutions for any application.

Solutions for your most difficult separations

We specialize in alternatives to reversed-phase for HPLC of proteins, peptides, and other biomolecules. 

Precise Separation of Charged Molecules

PolyLC’s ion exchange columns offer exceptional performance for the separation of charged molecules, including proteins, peptides, and monoclonal antibodies. Our columns are designed for high resolution, selectivity, and reproducibility, ensuring reliable results for your research.

Unmatched Expertise in HILIC Chromatography

As the inventors of HILIC, PolyLC offers unparalleled expertise and a wide selection of HILIC columns for the analysis of polar and hydrophilic compounds. Our columns deliver exceptional performance, reproducibility, and sensitivity, making them ideal for a variety of applications.

Preserve Protein Integrity and Activity

PolyLC’s HIC columns provide a gentle and effective method for separating biomolecules based on their hydrophobicity. Our columns are designed to maintain protein integrity and activity, ensuring optimal results for your research.

Size-Based Separation of Molecules

PolyLC’s SEC columns offer superior performance for the size-based separation of proteins, peptides, and amino acids. Our columns are designed for high resolution, accuracy, and reproducibility, making them ideal for a variety of applications.

Versatile & Efficient Purification

PolyLC’s Solid Phase Extraction (SPE) columns and pipette tips offer a streamlined approach to sample preparation, enabling efficient analyte concentration and purification.

Lab-Quality Analysis, Anywhere

PolyLC’s SmartLife LC™ is a compact, lightweight, and truly portable HPLC system, offering the power and performance of a traditional HPLC in a convenient, field-deployable format. With its user-friendly interface, customizable configurations, and long battery life, the SmartLife LC™ empowers scientists to conduct on-site analysis of hemoglobin samples with confidence and ease.

Ion Exchange Columns

Precise Separation of Charged Molecules

PolyLC’s ion exchange columns offer exceptional performance for the separation of charged molecules, including proteins, peptides, and monoclonal antibodies. Our columns are designed for high resolution, selectivity, and reproducibility, ensuring reliable results for your research.

HILIC Columns

Unmatched Expertise in HILIC Chromatography

As the inventors of HILIC, PolyLC offers unparalleled expertise and a wide selection of HILIC columns for the analysis of polar and hydrophilic compounds. Our columns deliver exceptional performance, reproducibility, and sensitivity, making them ideal for a variety of applications.

HIC Columns

Preserve Protein Integrity and Activity

PolyLC’s HIC columns provide a gentle and effective method for separating biomolecules based on their hydrophobicity. Our columns are designed to maintain protein integrity and activity, ensuring optimal results for your research.

SEC Columns

Size-Based Separation of Molecules

PolyLC’s SEC columns offer superior performance for the size-based separation of proteins, peptides, and amino acids. Our columns are designed for high resolution, accuracy, and reproducibility, making them ideal for a variety of applications.

Solid Phase Extraction

Versatile & Efficient Purification

PolyLC’s Solid Phase Extraction (SPE) columns and pipette tips offer a streamlined approach to sample preparation, enabling efficient analyte concentration and purification.

Portable HPLC Instrument

Lab-Quality Analysis, Anywhere

PolyLC’s SmartLife LC™ is a compact, lightweight, and truly portable HPLC system, offering the power and performance of a traditional HPLC in a convenient, field-deployable format. With its user-friendly interface, customizable configurations, and long battery life, the SmartLife LC™ empowers scientists to conduct on-site analysis of hemoglobin samples with confidence and ease.

Experience the PolyLC Advantage

Unmatched Expertise

With over 35 years of experience and a team of chromatography experts, we provide the knowledge and support you need to achieve your research goals.

Superior Quality

Our strict quality control standards minimize lot-to-lot variability, ensuring consistent product performance for reliable results.

Customized Solutions

We understand that every research project is unique. That’s why we offer personalized support and customization options to tailor our products to your specific needs.

Explore our Applications

Hemoglobin Analysis

Accurately separate and quantify hemoglobin variants with PolyLC’s specialized columns for reliable diagnostics and research.

Oligonucleotide Analysis

Ensure purity and integrity of oligonucleotides with PolyLC’s high-performance columns designed for precise separation.

Protein Purification

Achieve high-resolution separation and purification of proteins using PolyLC’s optimized chromatography solutions.

Peptide Isolation

Isolate individual peptides from crude extracts using two complementary modes of HPLC in sequence with PolyLC’s high-performance columns.

Looking for guidance on a specific application or method?

Check out our collection of application notes, posters, and chromatography resources.

Ready to experience the PolyLC difference?

Contact us to discuss your chromatography needs and let us help you find the optimal solution for your specific application.