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- for Hydrophilic Interaction and Size Exclusion HPLC -

This neutral, polar material was developed specifically for Hydrophilic Interaction Chromatography (HILIC). HILIC is a variant of normal phase chromatography that is performed with a polar stationary phase and a partly aqueous mobile phase. This permits normal-phase separations of peptides, nucleic acids, carbohydrates, some proteins, and polar solutes in general. In comparison with other materials used for HILIC, PolyHYDROXYETHYL A™ affords sharper peaks and better selectivity and recovery. Its great polarity means less organic solvent is needed to get retention. Use PolyHYDROXYETHYL A™ for:

1) Analysis of polar small molecules for metabolomics or analysis via HILIC-MS/MS of specific small molecules (amino acids; methotrexate) in plasma or crude extracts.

2) Peptide separations and mapping involving differences in polar groups (Ser-; glycopeptides; etc.).

3) Multidimensional purification of synthetic and natural peptides or fractionation of really complex digests.

4) HPLC of solutes that aren't soluble in aqueous media (membrane proteins; phospholipids).

5) Eliminating detergents, lipids, and salts from a sample.

6) Oligonucleotides and their analogs.

In the absence of organic solvent, PolyHYDROXYETHYL A™ functions in the Size Exclusion Chromatography (SEC) mode.

For proteins and peptides, use 200- or 300-Å material. For polar small solutes, try our premium 3-µm, 100-Å material.



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